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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in many tumors, which leads to chromosome instability, and thus might contribute to tumorigenesis. This protein was discovered to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial role in marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually explained. Two extra splice variants were described but thorough nucleotide sequence details is not really still obtainable.

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Blocking peptides are peptides that bind precisely towards the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope acknowledged with the antibody.

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This guidebook outlines even further probable optimization more info of mobile and immunoassay parameters to make sure the best possible final results are...

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This guide outlines further more attainable optimization of cellular and immunoassay parameters to be certain the absolute best results are...

Antibodies certain to the blocking peptide no longer bind to your epitope within the concentrate on protein. By comparing the staining with the blocked antibody versus the antibody by yourself, you can see which staining is certain.

Concentrate on Info Cyclin E belongs on the really conserved cyclin household, whose users are characterised by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which add towards the temporal coordination of each mitotic occasion. Cyclin E kinds a fancy with and features like a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates within the G1-S period boundary which is degraded as cells development as a result of S period.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...

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